Device

Part:BBa_K1329004:Design

Designed by: iGEM Marburg 2014   Group: iGEM14_Marburg   (2014-10-15)


NRPS: tRNA scaffold


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 207
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We determined the crystal structure - domain designed was structure based. No restriction sites had to be removed.


Source

Yes, genomic DNA from S. cerevisiae: Genebank: CAA64750.1

References